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KMID : 0361020070500090784
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Korean Journal of Otolaryngology - Head and Neck Surgery
2007 Volume.50 No. 9 p.784 ~ p.788
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Effect of Macrolide on the MUC4 Gene Expression in Human Airway Epithelial Cells
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Woo Hyun-Jae
Lee Seok-Choon
Choi Yoon-Seok
Choi Sung-Won
Kim Min-Han
Jeon Bo-Sung
Song Si-Youn
Kim Yong-Dae
Bae Chang-Hoon
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Abstract
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Background and Objectives: Mucin gene expression and mucin secretion are highly increased by inflammatory airway diseases such as asthma, chronic bronchitis and rhinosinusitis. Macrolide antibiotics is considered one of the effective drugs inhibiting mucus secretion for chronic bronchitis and rhinosinusitis with nasal polyp. However, the anti-secretory effect of macrolide is not clear. This study was designed to investigate whether macrolide can suppress interleukin-1beta (IL-1beta)-induced MUC4 gene expression and mucin secretion in the cultured human nasal polyp epithelial cells and NCI-H292 epithelial cells.
Subjects and Methods: Nasal polyps were obtained from 20 patients with chronic rhinosinusitis with nasal polyp during endoscopic sinus surgery. We observed the effect of roxithromycin on the IL-1beta-induced MUC4 gene and mucin secretion by reverse transcriptase-polymerase chain reaction (RT-PCR) with enzyme-linked immunosorbent assay (ELISA) method.
Results:Roxithromycin attenuated the IL-1beta-induced MUC4 mRNA expression and mucin secretion with a dose dependent pattern in both of the cultured human nasal polyp epithelial cells and NCI-H292 epithelial cells.
Conclusions:This result suggests that roxithromycin may be considered as an effective anti-hypersecretory agent for its down-regulation of the MUC4 gene.
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KEYWORD
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MUC4, Mucin, Roxithromycin
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